A flexible multiplexed immunosensor for point-of-care in situ wound monitoring
- May 21, 2021
A flexible multiplexed immunosensor for point-of-care in situ wound monitoring
Yuji Gao*, Dat T. Nguyen, Trifanny Yeo, Su Bin Lim, Wei Xian Tan, Leigh Edward Madden, Lin Jin, Ji Yong Kenan Long, Fazila Abu Bakar Aloweni, Yi Jia Angela Liew, Mandy Li Ling Tan, Shin Yuh Ang, Sivagame D/O Maniya, Ibrahim Abdelwahab, Kian Ping Loh, Chia-Hung Chen, David Laurence Becker, David Leavesley, John S. Ho and Chwee Teck Lim*
Abstract
Chronic wounds arise from interruption of normal healing due to many potential pathophysiological factors. Monitoring these multivariate factors can provide personalized diagnostic information for wound management, but current sensing technologies use complex laboratory tests or track a limited number of wound parameters. We report a flexible biosensing platform for multiplexed profiling of the wound microenvironment, inflammation, and infection state at the point of care. This platform integrates a sensor array for measuring inflammatory mediators [tumor necrosis factor–α, interleukin-6 (IL-6), IL-8, and transforming growth factor–β1], microbial burden (Staphylococcus aureus), and physicochemical parameters (temperature and pH) with a microfluidic wound exudate collector and flexible electronics for wireless, smartphone-based data readout. We demonstrate in situ multiplexed monitoring in a mouse wound model and also profile wound exudates from patients with venous leg ulcers. This technology may facilitate more timely and personalized wound management to improve chronic wound healing outcomes.
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